Inosine factory
Inosine factory
The production methods of Inosine mainly include chemical synthesis, enzymatic solution and fermentation. Chemical synthesis method due to its complex process, high equipment requirements, coupled with the large use of organic solvents there are raw material sources and environmental pollution problems, this method has been gradually eliminated in industrial production. Enzymatic solution is mainly based on RNA, by cutting off its 5'-OH and 3'OH bit binding of the phosphate diester bonds to achieve the purpose of producing nucleosides, but due to the decomposition process produced a large number of niconucleotides, and these substances have not been well used, resulting in waste of resources. At present, many fermentation methods are used at home and abroad to produce myoside, the remarkable feature of this method is low cost, high efficiency, the principle is that the use of strains with the ability to synthesize a specific nucleotide, through its mutagenic treatment, the selection of nutritional defects and nucleoside structure analogue resistance mutant strains, the removal of feedback inhibition and feedback inhibition in the metabolic process, can accumulate the desired products. After constructing a gene-engineered strain with high myosine yield, it is necessary to go through a strict bacteriological screening process in production, select the most dynamic and highest production metabolic capacity of the fine strains, and then go through two to three levels of seeds Amplification of culture, the seed liquid into fermentation tank for fermentation culture, in the process, it is necessary to strictly control the composition of seeds and fermentation media, especially carbon, nitrogen sources of rational use and matching, effectively control the direction of metabolic flow, as far as possible to inhibit the EMP pathway and enhance the HMP pathway, Provides a large amount of precursor substance PRPP (5-phosphate ribose-1-pyrophosphate) for myosine synthesis and a large amount of reducing force NADPH (also prototype coenzyme II) for product synthesis. In addition, it is necessary to use suitable pH, temperature, ventilation and other fermentation culture conditions, so that the bacteria in the optimal environment to maximize the accumulation of Inosines.
Chemwill Asia co.,Ltd is one of the leading factory of Organic compounds in CHINA.We own GMP licence as well as ISO certificate paper. CHemwill employs over 165 peoples including professional technical experts and professional-trained workers. The sales volume of our company in 2018 is over 8.5 million dollars. Our main production base is located in Xuzhou industry park. We produce a wide range of organics including Active pharmaceutical ingredients(APIs), Veterinary, Indole derivatives, Aromatics, Fluorine, Boronic acids,organocatalysts, chiral building blocks, heterocyclic compounds. We are certified both to the ISO 9001 and ISO 14001 Standards, have a safety management system in place. If you need,please contact us.